Circadian Analysis for Multi-Well Plate Readers
MultiCycle can read text-formatted data from any multi-well plate reader, including Tecan, ViewLux and Envision (Perkin Elmer), Synergy (Biotek), and LEADseeker (GE). The program calculates period, phase, amplitude, and damping for each well in a multi-plate trial and exports the parameters in spreadsheet form. Wells can be grouped into experimental and control groups for comparison. With flexible graphical displays (below), differences between control and experimental groups can be readily seen, and unusual samples immediately pop out.
The image below shows the analysis of the one plate in a 15-plate trial. The different groups were selected by clicking-and-dragging over the plate diagram to the left. A portion of the traces is selected with the vertical blue cursors, and the background subtraction method is selected (lower left). The resulting traces are plotted at the upper right. Oscillation parameters are automatically calculated, and period and phase are plotted against amplitude in the graphs below, color coded by group. Colors are user-selectable. The user has then clicked on one of the outlying points in the Period graph (red); and the corresponding point in the phase graph, the corresponding trace, and the corresponding well in the plate diagram are all automatically highlighted in red.
Data can be exported to spreadsheet-compatible text files for the entire trial, or for separately for plates and groups.
Traces can also be displayed in heat-map form (figure below). The well number for any sample in the heat-map can be identified by hovering the cursor over it.
MultiCycle is Windows XP/7/8/10 compatible or can be run in Parallels on Macintosh OSX.